My laboratory works towards a quantitative understanding of mammalian cellular metabolism, with a particular focus in cancer metabolism. We combine fluxomics and metabolomics with system biology, biochemistry and genetic approaches, to investigate how cellular metabolism is rewired to support cancer proliferation and metastasis, and how cellular metabolism is dynamically regulated in response to various microenvironments.
I will also join the Department of Nutritional Sciences as an assistant professor in summer 2017. My lab welcomes applications from prospective postdocs and graduate students.
Education
PhD, 2014, Princeton University
Selected Publications
- Fan J, Baeza J, Denu JM. (2016) Investigating histone acetylation stoichiometry and turnover rate. Methods Enzymol. 574:125-48.
- Dittenhafer-Reed KE*, Richards AL*, Fan J, Smallegan MJ, Siahpirani AF, Kemmerery ZA, Prolla TA, Roy S, Coon JJ, Denu JM. (2015) SIRT3 mediates multi-tissue coupling for metabolic fuel switching. Cell Metab. 21(4):637-46
- Fan J*, Teng X*, Liu L, Mattaini KR, Looper RE, Vander Heiden MG, Rabinowitz JD Human Phosphoglycerate Dehydrogenase Produces the Oncometabolite d-2-Hydroxyglutarate. (2015) ACS Chem. Biol. 10(2):510-6.
- Fan J, Krautkramer KA, Feldman JL, Denu JM. (2015) Metabolic regulation of histone
post-translational modifications. ACS Chem. Biol. 10(1):95-108 - Zhang J, Fan J, Venneti S, Cross JR, Takagi T, Bhinder B, Djaballah H, Kanai M, Cheng EH, Judkins AR, Pawel B, Baggs J, Cherry S, Rabinowitz JD, Thompson CB. (2014) Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion. Mol. Cell. 56(2):205-18
- Fan J*, Ye J*, Kamphorst JJ, Shlomi T, Thompson CB, Rabinowitz JD. (2014) Quantitative flux analysis reveals folate-dependent NADPH production. Nature. 510(7504):298-302.
Ye J*, Fan J*, Venneti S, Wan YW, Pawel BR, Zhang J, Finley LW, Lu C, Lindsten T,
Cross JR, Qing G, Liu Z, Simon MC, Rabinowitz JD, Thompson CB. (2014) Serine
catabolism regulates mitochondrial redox control during hypoxia. Cancer Discov.
4(12):1406-17 - Fan J, Kamphorst JJ, Mathew R, Cheung MK., White E, Shlomi T, Rabinowitz JD. (2013) Simultaneous aerobic glycolysis and glutamine-supported oxidative ATP production in oncogene-driven renal epithelial cells. Mol. Syst. Biol. 9: 712.
- Fan J, Kamphorst JJ, Rabinowitz JD, Shlomi T. (2013) Fatty acid labeling from glutamine in hypoxia can be explained by isotope exchange without net reductive IDH flux. J. Biol. Chem. 288(43):31363-9
- Kamphorst JJ*, Cross JR*, Fan J, De Stanchina E, Mathew R, White E, Thompson CB, Rabinowitz JD. (2013) Hypoxia and oncogenic Ras trigger scavenging of fatty acids from lysophospholipids. Proc. Nat. Acad. Sci. 110(22):8882-7
- Guo JX*, Chen HY*, Mathew R*, Fan J*, Strohecker AM, Karsli-Uzunbas G, Kamphorst JJ,Chen G, Lemmons JM, Karantza V, Coller HA, DiPaola RS, Gelinase C, Rabinowitz, JD, White E. (2011) Activated Ras Requires Autophagy to Maintain Oxidative Metabolism and Tumorigenesis. Genes Dev. 25(5):460-70